Lecturer(s): Emmanuelle LAURENCEAU
Course ⋅ 4 hPW ⋅ 7 hAutonomy ⋅ 2 hStudy ⋅ 4 h


This course will allow engineering students to identify the stages of production of a recombinant protein as well as the different purification methods, their roles and interests in bioproduction processes. The production of recombinant proteins by genetic engineering methods is a common process in most areas of biotechnology. Using perfectly mastered methods, this process makes it possible to obtain specific proteins, in particular of therapeutic interest, with a very high yield.


Course (4h):

  • Principle of genetic engineering
  • Production and purification of recombinant protein

BE (4h): Biofermenter

Practical (7h): Microbrewery

Learning Outcomes

  • Know the techniques of bio-production and characterization of biomolecules
  • Set up an experimental protocol
  • Present results in a relevant, rigorous and critical manner for analysis
  • Write a complete technical report, correctly referenced


50% knowledge (course exam and BE), 50% know-how (practical report)