Objectives
This course will allow engineering students to identify the stages of production of a recombinant protein as well as the different purification methods, their roles and interests in bioproduction processes. The production of recombinant proteins by genetic engineering methods is a common process in most areas of biotechnology. Using perfectly mastered methods, this process makes it possible to obtain specific proteins, in particular of therapeutic interest, with a very high yield.
Programme
Course (4h):
- Principle of genetic engineering
- Production and purification of recombinant protein
BE (4h): Biofermenter
Practical (7h): Microbrewery
Learning Outcomes
- Know the techniques of bio-production and characterization of biomolecules
- Set up an experimental protocol
- Present results in a relevant, rigorous and critical manner for analysis
- Write a complete technical report, correctly referenced
Assesment
50% knowledge (course exam and BE), 50% know-how (practical report)